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. 2020 Jan 16;34(3):4147–4162. doi: 10.1096/fj.201902534R

Figure 7.

Figure 7

TPC2 knockdown attenuates Tat endolysosome escape. A, Quantitative immunoblotting data showed that the expression of TPC2 was knocked down with specific shRNAs in H1299 cells (n = 3; **P < .01). B, Knockdown of TPC2 significantly attenuated Tat endolysosome escape in the absence of chloroquine (n = 3; *P < .05). Blocking TPCs with Ned‐19 decreased Tat endolysosome escape in control shRNA‐treated cells but did not further decrease Tat endolysosome escape in TPC2 knockdown cells (n = 3; *P < .05). C, TPC2 knockdown significantly enhanced the internalization of Tat‐FITC (n = 4, *P < .05, ***P < .001, bar = 10 μm). D, Quantitative immunoblotting data showed that the expression of TRPML1 was knocked down with specific shRNAs in H1299 cells (n = 3; **P < .01). E, Knockdown of TRPML1 did not affect Tat endolysosome escape (n = 3; P > .05)