Figure 2.

Predicting secondary structure of aptamers. A, Predicted secondary structures of the top nine list of aptamers (#1‐9) with ranking according to AptaSUITE cluster family size. Structures were predicted using UNAFold software. Manually mapped DUX4 motifs are highlighted in red (forward) and blue (reverse). B, The amount of structure elements bulge loops, hairpin loops, interior loops, stem parts, and unpaired ends were depicted by their occurrence on aptamer position 1 to 45 of the selected 350 aptamers. C, Visualization of the amount of structural elements as logo: E unpaired ends, H hairpin loops, I interior loops, L bulge loops, S stem parts