Figure 4.

Testing the binding of SELEX‐originated aptamers. Comparing top nine list of SELEX generated aptamers in TR‐FRET assays. Aptamers were compared by competing against the 5′‐end Atto647N‐labeled template aptamer after incubation with StrepII‐SUMO‐DUX4‐His6. The fitted curves are displayed together with the IC50 values in micromolar from single experiments with ±95% confidence interval (bar plot). The SELEX aptamers were sorted according to their cluster size in counts‐per‐million (cpm) based on AptaSUITE software preprocessing steps (values in brackets). NA are not‐fittable data points indicating IC50 values higher than 10 µM