Figure 9.

TR‐FRET competition assay was performed to compare the affinity of a template DUX4 aptamer with chemical loop modifications. An unmodified triple‐dC loop and an aptamer without loops were compared to aptamers with loop lacking the nucleobases (abasic) and aptamer with loops having aliphatic C3 spacer (no deoxyribose) between the phosphates. The chemical structures of the loops are depicted below. Single experiment data is shown as IC50 ± standard error of fitting to a four‐parameter logistic using R package “drc”. Curves are provided in Supplemental Figures S4D