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. 2019 Oct 3;75(3):648–659. doi: 10.1111/all.14036

Figure 2.

Figure 2

Alum promotes the generation of Th1, Th2 and Th17 cells and impairs the induction of IL‐10‐producing T cells by PM. A and B, Cytokines (A) or cytokine ratios (B) produced by allogeneic naïve CD4+ T cells primed by PM‐ or PM and alum‐activated hmoDCs from healthy donors after 5 d (n = 6). C, After 5 d of coculture, primed T cells were washed and stimulated for 6 h with PMA/ionomycin in the presence of brefeldin A. Percentage of CD3+CD4+ T cells producing the indicated cytokines after intracellular staining and flow cytometry analysis (n = 4). D, Representative dot plots for the intracellular staining after flow cytometry analysis. E and F, Cytokines (E) or cytokine ratios (F) produced by allogeneic naïve CD4+ T cells primed by PM‐ or PM and alum‐activated total blood DCs from healthy donors after 5 d (n = 5). Paired Student t test: *P < .05 and ** P < .01