Figure 3.

Alum reduces PM‐induced functional FOXP3⁺ Treg cells. A, Percentage of induced CD4⁺CD25^highCD127^‒FOXP3⁺ Treg cells by allogeneic medium (Ctrl ‐)‐, alum‐, PM‐ or PM and alum‐treated hmoDCs from healthy donors after 5 d (gating in lymphocytes, n = 9). B, Proliferation of CFSE‐labelled PBMCs gated on CD4⁺ T cells after 5 d of coculture with autologous purified FOXP3⁺ Treg cells generated by allogeneic PM‐ or PM and alum‐treated hmoDCs (ratio 1:1). The percentage of proliferating responder PBMCs stimulated with anti‐CD3 and anti‐CD28 for each condition at a 1:1 ratio is shown (n = 2). C, CD4⁺CD25^highCD127^‒FOXP3⁺ Treg cells generated by allogeneic medium (Ctrl ‐)‐, alum‐, PM‐ or PM and alum‐treated total blood DCs after 5 d (gating in lymphocytes, n = 4). Paired Student t test: *P < .05, **P < .01 and ***P < .001