Figure 2.

Cytotoxic and effector molecules in CD28⁺CD57⁻, DP, DN, and CD28⁻CD57⁺ cells within CCR7⁻CD8⁺ T cells. (A) Complete gating strategy used to define the populations of interest in the experiments performed using flow cytometry. After gating on CD3⁺CD8⁺ T cells within lymphocytes, effector (CCR7⁻) cells and the CD28⁺CD57⁻, DP, DN, and CD28⁻CD57⁺ subsets were defined. Frequency of CX3CR1⁺ (B), TNF⁺ (C), IFN‐γ⁺ (D), and IL‐10⁺ (E) cells within CD28⁺CD57⁻, DP, DN, and CD28⁻CD57⁺ CCR7⁻CD8⁺ T cells in PBMCs obtained from healthy donors. Representative flow cytometry plots reporting the expression of each molecule within the DP subset are shown. N = 21 for each graph. Data are shown as mean ± SEM and pooled from 14 independent experiments. One‐way ANOVA, Bonferroni post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001.