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. 2020 Mar 6;2020:6724810. doi: 10.1155/2020/6724810

Figure 2.

Figure 2

The effects of TRPM7 on hypoxia-induced EMT in androgen-independent prostate cancer cells. (a, b) PC3 (a) and DU145 cells (b) were grown in either hypoxic condition (H) or normoxic condition (N) for 24 h. TRPM7 protein and EMT markers (E-Cad, N-Cad, and vimentin) were determined using western blotting. The representative images and the densitometry analyzed results were shown. ∗ versus normoxia group, p < 0.05, n = 4. (c, d) PC3 (c) and DU145 cells (d) were carried out to knockdown TRPM7 by TRPM7 siRNA (Si-T7), and negative siRNA (Si-Con) was used as control. Cells were suffered hypoxia for 24 h. TRPM7 protein and EMT markers (E-Cad, N-Cad, and vimentin) were determined using western blotting. The representative images and the densitometry analyzed results were shown. ∗ versus negative control siRNA group, p < 0.05, n = 5.