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. 2020 Mar 6;2020:6724810. doi: 10.1155/2020/6724810

Figure 4.

Figure 4

TRPM7 knockdown restored the phosphorylation of RACK1 (p-RACK1), while enhanced the interaction between RACK1 and HIF-1α in PC3 cells. (a) Western blotting results showed the protein expression of p-RACK1, RACK1, and HSP90 in PC3 prostate cancer cells under normoxic (N), hypoxic conditions (H), and hypoxia plus siRNA control (H + Si − Con) or siRNA-TRPM7 (H + Si − T7) for 24 h. ∗, # versus N and H + Si − Con, respectively, p < 0.05, n = 6. (b) Co-IP of HIF-1α with RACK1 and HSP90 after TRPM7 knockdown in PC3 prostate cancer cells under hypoxic condition. ∗ versus H + Si − Con, p < 0.05, n = 4. (c) Co-IP of HIF-1α with RACK1 and HSP90 after RACK1 knockdown in PC3 prostate cancer cells under hypoxic condition. ∗ versus H + Si − Con, p < 0.05, n = 4.