KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rb anti Histone H3 (acetyl K27) | Abcam | RRID:AB_2118291 |
| Ms anti Human/Mouse UCP1 | R&D | RRID: AB_10572490 |
| Ms anti Alpha tubulin | Sigma | RRID:AB_477583 |
| Ms anti Total Oxphos Rodent Antibody Cocktail | Abcam | RRID:AB_2629281 |
| Anti-rabbit IgG IRDye 800CW | Li-Cor | RRID:AB_621848 |
| Anti-mouse IgG IRDye 800CW | Li-Cor | RRID:AB_621847 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| BCS | ATCC | Cat#30–2030 |
| HEPES 1M | Thermo Fisher | Cat#15630080 |
| 16% Paraformaldehyde | EMS | Cat#15710 |
| Protein-A Sepharose CL-4B Beads | GE Healthcare | Cat#17–0780-01 |
| PCR Master Mix, Power SYBR Green | Applied Biosystems | Cat#4367659 |
| Lipofectamine 2000 | Invitrogen | Cat#11668027 |
| NEBNext Ultra II DNA Library Prep with Sample Purification Beads | NEB | Cat#E7103 |
| NEB Next Ultra RNA Library Prep Kit | NEB | Cat#E7530 |
| DMEM | MediaTech | Cat#MT10–017-CV |
| Dispase II | Roche | Cat#4942078001 |
| Collagenase D | Roche | Cat# 11088882001 |
| TRIzol | Invitrogen | Cat#15596018 |
| Purelink RNA Mini columns | Invitrogen | Cat#LT-12183018 |
| ABI High-Capacity cDNA Synthesis kit | Applied Biosystems | Cat#4368813 |
| FluoroBrite DMEM | Thermo Fisher | Cat# A1896701 |
| CL-316,243 | Sigma Aldrich | Cat#C5976 |
| Free Glycerol Reagent | Sigma Aldrich | Cat#F6428 |
| Bovine Serum Albumin, Fraction V, Fatty Acid Free | Roche | Cat#03117057001 |
| Pierce BCA Protein Assay | Thermo Fischer | Cat#23225 |
| Deposited Data | ||
| RNA sequencing data | GEO repository | GSE144188 |
| H3K27Ac ChIP Sequencing data | GEO repository | GSE144188 |
| Experimental Models: Organisms/Strains | ||
| Adipoq-Cre | The Jackson Laboratory | RRID:IMSR_JAX:010803 |
| Ebf2 fl/fl | Dr. Patrick Seale | N/A |
| Ebf1 fl/fl | Dr. Rudolph Grosschedl | N/A |
| Recombinant DNA | ||
| pcDNA-PGC1 α, and pcDNA-ERRa | Dr. Mitchell Lazar | N/A |
| pcDNA-EBF2, pcDNA-EBF1, pcDNA-EBF2, pcDNA-EBF3, pcDNA-PPARγ, pGL4-Ucp1 −6, and pcDNA-RXRα | Dr. Patrick Seale | N/A |
| lentiCRISPR v2 | Addgene (F. Zhang) | RRID:Addgene_52961 |
| pMD2.G | Addgene (D. Trono) | RRID:Addgene_12259 |
| psPAX2 | Addgene (D. Trono) | RRID:Addgene_12260 |
| Software and Algorithms | ||
| GraphPad Prism 7 | Graphpad (www.graphpad.com/scientific-software/prism/ | RRID:SCR_002798 |
| UCSC Genome Browser | UCSC (http://genome.ucsc.edu/) | RRID:SCR_005780 |
| Morpheus (Heatmaps) by the Broad Institute | https://software.broadinstitute.org/morpheus/ | RRID:SCR_017386 |
| Homer | http://homer.ucsd.edu/ | RRID:SCR_010881 |
| Star Aligner | https://github.com/alexdobin/STAR | RRID:SCR_015899 |
| ToppGene | https://toppgene.cchmc.org/ | RRID:SCR_005726 |