Fig. 6.

Dynorphin/KOR regulated microglial M2 polarization inhibited neuronal apoptosis. The conditioned medium of BV-2 cells in the groups of Control, LPS, LPS + Dynorphin-A, LPS + GNT1, and LPS + Dynorphin-A + GNT1 were collected and co-cultured with the primary mouse hippocampal neurons. a The neuronal viability was examined by CCK-8 assay. b The neuronal apoptosis was examined by flow cytometry with Annexin-V-FITC/PI dual-labeling. c The protein levels of apoptosis-related caspase-3, Bax, and Bcl-2 in the primary mouse hippocampal neurons were examined by western blot. *p < 0.05, **p < 0.01, vs. Control group, ^#p < 0.05, ^##p < 0.01, vs. LPS group; ^&p < 0.05, ^&&p < 0.01, vs. LPS + Dyn-A group