Fig. 2.

Overexpressed CASC2 suppresses hypoxia-induced PASMC proliferation and migration. PASMCs were transfected with CASC2 or si-CASC2 prior to exposure to hypoxia (3% O₂) or normoxia for 24 h in triplicate. a, b Expression of CASC2 was detected by qRT-PCR in hypoxia- or normoxia-induced PASMCs. c, d Proliferation was determined using CCK-8 assay, and data were derived from an average of three independent experiments. e, f Wound healing assay was used to detect hypoxia-induced PASMC migration, and experiments were performed in triplicate. **P < 0.01, ***P < 0.001