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. 2020 Mar 17;25:21. doi: 10.1186/s11658-020-00215-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — ING5 is a target of miR-222. a Potential binding sites between miR-222 and ING5 were exhibited. b Enrichment of ING5 and miR-222 was detected in PASMCs after Ago2 RIP. c Luciferase activity was analyzed in PASMCs co-transfected with ING5-WT or ING5-MUT and miR-NC, miR-222 or anti-miR-NC, anti-miR-222. d ING5 protein expression in PASMCs transfected with miR-222 or anti-miR-222 was examined three times by western blot assay. **P < 0.01, ***P < 0.001