Fig. 6.

MiR-222 promotes hypoxia-induced PASMC proliferation and migration by regulating ING5 expression. a mRNA expression of ING5 was detected using qRT-PCR in PASMCs following O₂ exposure (21, 10, 3 and 1%) for 24 h. b ING5 mRNA expression in PASMCs exposed to 3% O₂ for 0 h, 12 h, 24 h, 36 h and 48 h was measured by qRT-PCR. c Protein expression of ING5 was determined by western blot assay in hypoxia-induced PASMCs after transfection with miR-NC, miR-222, miR-222 + pcDNA or miR-222 + ING5, protein bands were quantified and a bar chart showing relative protein levels was derived from triplicate samples. d CCK-8 assay was performed to determine the proliferation of hypoxia-induced PASMCs after transfection; data are derived from three independent experiments. e Migration ability of hypoxia-induced PASMCs was analyzed using wound healing assay, and results were presented as the average of three independent replicates. *P < 0.05, **P < 0.01, ***P < 0.001