Fig. 7.

CASC2 can regulate ING5 expression by directly binding to miR-222. a, c Expression of miR-222 and ING5 protein in PASMCs transfected with pcDNA, CASC2, CASC2 + miR-NC or CASC2 + miR-222 was detected using qRT-PCR or western blot, respectively. b, d Levels of miR-222 and ING5 protein in PASMCs transfected with si-NC, si-CASC2, si-CASC2 + anti-miR-NC or si-CASC2 + anti-miR-222 were examined by qRT-PCR or western blot, respectively. The same experiment was repeated three times, and the average was taken. **P < 0.01, ***P < 0.001