Fig. 8.

CASC2 can exert its inhibitory effect on proliferation and migration of hypoxia-induced PASMCs by regulating ING5 expression. PASMCs were transfected with si-NC, si-CASC2, si-CASC2 + pcDNA or si-CASC2 + ING5 in triplicate. a Protein expression of ING5 was measured by western blot in hypoxia-induced PASMCs after transfection, and the average from three independent experiments was taken. b CCK-8 assay was carried out to determine the proliferation of hypoxia-induced PASMCs after transfection, and all experiments were repeated three times independently. c Migration ability of hypoxia-induced PASMCs after transfection was analyzed using wound healing assay, and data are derived from an average of three independent experiments. **P < 0.01