Fig. 6.

miR-33a-mediated suppressive effects on malignant behaviors in TNBC cells can be offset by EZH2 overexpression. a, b The expression of EZH2 in TNBC cells after indicated transfected was determined by Western blot and qRT-PCR assays. a Representative blots and b the levels of EZH2 mRNA after in cells indicated transfections are shown. c–e Ectopic expression of EZH2 counteracted miR-33a-mediated reduction of EdU incorporation in TNBC cells. Representative images (c) and the number of EdU-positive MDA-MB-231 (d) and BT-549 (e) cells are shown. f, g EZH2 overexpression promoted the proliferation rate in MDA-MB-231 and BT-549. h, i Increased EZH2 reduced the percentage of cells arrested in G1 cell cycle mediated by miR-33a. j, k MiR-33a-induced decreases in cell migration and invasion capacities can be enhanced by ectopic expression of EZH2. l EZH2 overexpression promoted the secret of MMP9 in TNBC cells. Data are shown as mean ± SEM (n = 3). *P < 0.05, **P < 0.01 and ***P < 0.001 compared with cells transfected with NC + Vector groups; ^#P < 0.05, ^##P < 0.01 and ^###P < 0.001 compared with cells transfected with miR-33a mimic groups