Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Mar 17;11:123. doi: 10.1186/s13287-020-01634-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 1 — Non-adherent 3D-cultured prostatospheroids exhibit increased expressions of two PCSC-associated surface markers EpCAM and CD44. a Representative images of three selected prostate cancer cell lines (AR-positive, VCaP and LNCaP; AR-negative, DU145) grown under the adherent 2D-culture condition (magnification, × 20; bars, 100 μm) and the non-adherent 3D-culture on agar-coated surfaces (magnification, × 20; bars, 200 μm). b RT-qPCR and immunoblot analyses of EpCAM and CD44. Results showed that all prostatospheroids derived from different prostate cancer cells expressed significantly higher mRNA and protein levels of EpCAM and CD44 as compared to their counterpart adherent cultured cells. *P < 0.05 versus adherent cultured cells