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. 2020 Mar 17;11:123. doi: 10.1186/s13287-020-01634-6

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Detection of apoptotic cells in prostatic cells upon 1 h incubation with P-DC-CIK or NP-DC-CIK by TMR red-based TUNEL assay. a Representative images show the TUNEL-positive apoptotic cells induced in prostatospheroid-derived prostate cancer cells. b Graph shows the quantitation of TMR red signals detected in prostatic cells upon co-cultures with P-DC-CIK or NP-DC-CIK cells. Results showed that significant increases of TMR red-labeled apoptotic cells were detected in prostatospheroid-derived prostate cancer cells (DU145, LNCaP, and VCaP) upon co-culture with the P-DC-CIK as compared to the NP-DC-CIK control. Results also showed that no TMR red-labeled apoptotic cells were detected among BPH-1 immortalized non-malignant prostatic epithelial cells upon incubation with either P-DC-CIK or NP-DC-CIK. Magnification, × 20; bars, 100 μm. NP-DC-CIK non peptide-sensitized dendritic cells-cytokine-induced killer cell preparation, P-DC-CIK peptide-sensitized dendritic cells-cytokine-induced killer cell preparation