Table 1.
Antibody | Source | Reference | Type | Use | Dilution |
---|---|---|---|---|---|
Aquaporin-4 | Sigma-Aldrich | A5971 | RbP | FR | 1:200 |
BDNF | Abcam | ab108319 | RbM | I, VB | 1:250 |
CD11b | BioLegend | 101225 | RtM | FC | 1:80 |
CD34 | BioLegend | 119309 | MM | FC | 1:25 |
CD44 | BioLegend | 103015 | MM | FC | 1:50 |
CD45.1 | BioLegend | 110713 | MM | FC | 1:80 |
CD73 | BD | 561543 | RtM | FC | 1:25 |
CD90.2 | BioLegend | 105315 | RtM | FC | 1:200 |
Collagen type II | Calbiochem-Novabiochem | AB746P | RbP | C | 1:40 |
F4/80 | Biolegend | 123107 | RtM | I, FC | 1:200 |
GAPDH | Abcam | Ab9485 | RbP | WB | 1:2500 |
GDNF | Santa Cruz | sc-328 | RbP | I, FC, VB | 1:100 |
GFAP | Sigma | G-A-5 | MM | I, FC, VB, WB | 1:1000 |
δGFAP | Merck | AB9598 | RbP | I | 1:500 |
Glutamine synthetase | Abcam | Ab49873 | RbP | VB | 1:5000 |
Iba1 | Wako | 019-19741 | RbP | FR, VB, WB | 1:500, 1:1000 (WB) |
Myeloperoxidase | Abcam | Ab139748 | RbP | VB | 1:100 |
Nestin | BIO-RAD | AHP1739 | RbP | I, FC, VB | 1:100 |
NeuN | Merck | MAB377 | MM | I, FC, VB | 1:100 |
NG2 | Merck | AB5320 | RbP | I | 1:200 |
NGF | Abcam | AB6199 | RbP | I, VB | 1:500 |
RFP | Chromotek | 5F8 | RtM | FC, VB | 1:500 |
β-III tubulin | Promega | A6712 | MM | I, FC | 1:5000 |
VEGF | Abcam | Ab46154 | RbP | I, FC, VB | 1:1000 |
Abbreviations: C BM-MSC in cell cultures, I immunofluorescence for BM-MSC on slides, FC flow citometry, FR frozen sections, MM mouse monoclonal, RbM rabbit monoclonal, RtM rat monoclonal, RP rabbit polyclonal, VB vibratome sections, WB western blot. Sources: AbCam, Cambridge, UK; BD, San Diego, CA, USA; BioLegend, San Diego, CA, USA; BIO-RAD, Oxford, UK; Chromotek, Planegg-Martinsried, Germany; GeneTex, Irvine, CA, USA; Merck Millipore, Burlington, MA, USA; Promega, Madison, WI, USA; Santa Cruz Biotechnology, Dallas, TX, USA; Sigma-Aldrich