Skip to main content
. 2020 Mar 17;11:121. doi: 10.1186/s13287-020-01626-6

Table 1.

Primary antibodies

Antibody Source Reference Type Use Dilution
Aquaporin-4 Sigma-Aldrich A5971 RbP FR 1:200
BDNF Abcam ab108319 RbM I, VB 1:250
CD11b BioLegend 101225 RtM FC 1:80
CD34 BioLegend 119309 MM FC 1:25
CD44 BioLegend 103015 MM FC 1:50
CD45.1 BioLegend 110713 MM FC 1:80
CD73 BD 561543 RtM FC 1:25
CD90.2 BioLegend 105315 RtM FC 1:200
Collagen type II Calbiochem-Novabiochem AB746P RbP C 1:40
F4/80 Biolegend 123107 RtM I, FC 1:200
GAPDH Abcam Ab9485 RbP WB 1:2500
GDNF Santa Cruz sc-328 RbP I, FC, VB 1:100
GFAP Sigma G-A-5 MM I, FC, VB, WB 1:1000
δGFAP Merck AB9598 RbP I 1:500
Glutamine synthetase Abcam Ab49873 RbP VB 1:5000
Iba1 Wako 019-19741 RbP FR, VB, WB 1:500, 1:1000 (WB)
Myeloperoxidase Abcam Ab139748 RbP VB 1:100
Nestin BIO-RAD AHP1739 RbP I, FC, VB 1:100
NeuN Merck MAB377 MM I, FC, VB 1:100
NG2 Merck AB5320 RbP I 1:200
NGF Abcam AB6199 RbP I, VB 1:500
RFP Chromotek 5F8 RtM FC, VB 1:500
β-III tubulin Promega A6712 MM I, FC 1:5000
VEGF Abcam Ab46154 RbP I, FC, VB 1:1000

Abbreviations: C BM-MSC in cell cultures, I immunofluorescence for BM-MSC on slides, FC flow citometry, FR frozen sections, MM mouse monoclonal, RbM rabbit monoclonal, RtM rat monoclonal, RP rabbit polyclonal, VB vibratome sections, WB western blot. Sources: AbCam, Cambridge, UK; BD, San Diego, CA, USA; BioLegend, San Diego, CA, USA; BIO-RAD, Oxford, UK; Chromotek, Planegg-Martinsried, Germany; GeneTex, Irvine, CA, USA; Merck Millipore, Burlington, MA, USA; Promega, Madison, WI, USA; Santa Cruz Biotechnology, Dallas, TX, USA; Sigma-Aldrich