Table 4.
Upregulated proteins secreted by BM-MSC under TNFα stimulation compared with control C1
| Protein | Biological processes | Abundance ratio (sample/Control C1) | P value |
|---|---|---|---|
| C-X-C motif chemokine 3 | 1, 2, 3, 4, 5 | 11.38 | 1 × 10−1 |
| C-type lectin domain family 4 member E | 3,4, 5, 6 | 8.464 | 1 × 10−17 |
| Platelet factor 4 | 1, 3, 4, 7 | 5.818 | 5.54 × 10−10 |
| C-X-C motif chemokine 10 | 1, 3, 4, 5 | 5.198 | 2.44 × 10−12 |
| Pentraxin-related protein PTX3 | 3, 4, 5 | 4.613 | 5.66 × 10−11 |
| Stromelysin-1 | 4, 5, 8 | 3.936 | 4.98 × 10−09 |
| Isoform 2 of sequestosome-1 | 4, 5, 6, 7, 8, 9, 10 | 3.842 | 9.45 × 10−09 |
| Neutrophil gelatinase-associated lipocalin | 3, 4, 5, 7, 9, 10, 11 | 3.267 | 0.0002 |
| CD82 antigen | 4, 5 | 2.869 | 3.98 × 10−05 |
| Tumor necrosis factor receptor superfamily member 1B | 3, 4, 5, 12, 13 | 2.762 | 0.0006 |
| Copper transport protein ATOX1 | 2, 5, 11 | 2.572 | 7.45 × 10−05 |
| Prostaglandin G/H synthase 2 | 3, 4, 5, 6, 8 | 2.421 | 0.005 |
| Intercellular adhesion molecule 1 | 1, 2, 3, 4, 5, 7, 9 | 2.107 | 0.002 |
| GRIP1-associated protein 1 | 4, 8, 11 | 2.023 | 0.02 |
| MAGUK p55 subfamily member 6 | 8 | 2.013 | 0.03 |
Mass spectrometry analysis of secretome. The P value is calculated in a background-based ANOVA. This method uses the background population of ratios for all peptides and proteins to determine whether any given single peptide or protein is significantly changing relative to that background. Biological processes: 1, cellular component movement; 2, cellular homeostasis; 3, defense response; 4, regulation of biological process; 5, response to stimulus; 6, cell differentiation; 7, cell organization and biogenesis; 8, metabolic process; 9, cell communication; 10, cell death; 11, transport; 12, cell proliferation; 13, development. More data of these proteins is shown in Additional file 5