Table 3.
Classification of genes used in this study from C. suis and their comparison with other coccidian parasites
| Cystoisospora suis | Toxoplasma gondii | Eimeria necatrix | Neospora caninum | ||||
|---|---|---|---|---|---|---|---|
| Accession no. | Accession no. | Identity (%) | Accession no. | Identity (%) | Accession no. | Identity (%) | |
| Dynein light chain type 1 (DLC1) | CSUI_000751 | TGARI_244900 | 71.70 | ENH_00055140 | 36.49 | NCLIV_019360 | 74.53 |
| Male gamete fusion factor (HAP2) | CSUI_000472 | TGARI_285940 | 63.80 | ENH_00067440 | 37.5 | NCLIV_014480 | 61.28 |
| Oocyst Wall protein (OWP1) | CSUI_006207 | TGARI_204420 | 65.87 | ENH_00062180 | 23.45 | NCLIV_020820 | 66.87 |
| Tyrosine rich, “Eimeria gam-like” | CSUI_001473 | TGARI_237080 | 54.97 | ENH_00047090 | 22.52 | NCLIV_050960 | 48.83 |
| DNA repair proteinRad51/dmc1-like | CSUI_004539 | TGARI_272900 | 93.18 | ENH_00059490 | 81.98 | NCLIV_059840 | 46.23 |
| Nima-related protein kinase 1 Nima1 | CSUI_004317 | TGARI_292140A; TGARI_292140B | 43.97 | ENH_00060280 | 46.41 | NCLIV_043340 | 42.44 |
| Nima-related protein kinase 2 Nima2 | CSUI_003099 | TGARI_307640 | 86.62 | ENH_00076430 | 65.94 | ||
| Nima-related protein kinase 4 Nima4 | CSUI_000744 | TGARI_244620 | 77.14 | ENH_00003390 | 31.27 | NCLIV_019170 | 83.46 |
Notes: Percentage values represent identities of Toxoplasma, Eimeria and Neospora genes with their corresponding Cystoisospora orthologs. All sequences are found in the genomic resource database ToxoDB (https://toxodb.org/toxo/). Alignment analyses were performed using the Clustal omega (https://www.ebi.ac.uk/Tools/msa/clustalo)