Fig. 4.

Gut-trafficking blockade increases colitis severity under the condition of checkpoint blockade. a The experimental design for the whole process. b, c WT mice were given 3.5% DSS for 7 days and treated without CTLA-4 blockade (IgG as the isotype control). Percent of the initial weight of mice receiving the IgG isotype control (Iso Ctrl) or anti-β7 mAb (b). Survival of the mice receiving the IgG isotype control (Iso Ctrl) or anti-β7 mAb (c). d, e WT mice were given 3% DSS for 7 days and treated with CTLA-4 blockade. Percent of the initial weight of mice receiving the IgG isotype control (Iso Ctrl) or anti-β7 mAb (d). Survival of the mice receiving the IgG isotype control (Iso Ctrl) or anti-β7 mAb (e). There were five mice in each group. The data are shown as the mean and SEM determined by two-way ANOVA with Sidak’s correction for multiple comparisons, ***P < 0.001. Survival was monitored for 20 days