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. 2020 Mar 18;16:40. doi: 10.1186/s13007-020-00582-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Dataset preparation: a LemnaTec image (of size 6576 × 4384) consists of not only the plant regions but also the chamber used in imaging; b the images are cropped (of size 1656 * 1356) to get only the region of interests; c spike pixels (x, y) are selected and previous color of the selected pixel is replaced by the fill_color; d segmented mask image is generated by setting the pixel value of fill_color as 0 (black) elsewhere, 255 (white)