Table 1.
Primers used in this study*
| Gene | Forward Primer (5'->3') | Reverse Primer (5'->3') | |
|---|---|---|---|
| Adipogenic | PPARγ | GAGGCAGCAGAGGTTAACAGA | CACCGAGGCGTAAAGTACCA |
| LPL | CCGCCGACCAAAGAAGAGAT | TAGCCACGGACTCTGCTACT | |
| FABP4 | TGGGCCAGGAATTTGACGAA | CACATGTACCAGGACACCCC | |
| Osteogenic | RUNX2 | CCTACCTGAGCCAGATGACG | ATGCTGGGTGGCCTGAAAT |
| ALP | GAATCTTCCCCAAGGGCCAA | CAGAATGTTCCACGGAGGCT | |
| BGLAP | TCCTTTGGGGTTTGGCCTAC | CCAGCCTCCAGCACTGTTTA | |
| Chondrogenic | SOX9 | AGGAAGTCGGTGAAGAACGG | AAGTCGATAGGGGGCTGTCT |
| COL2 | GCTCCTGCCGTTTCGCTG | ATTATACCTCTGCCCATCCTGC | |
| ACAN | CTTCCGCTGGTCAGATGGAC | CGTTTGTAGGTGGTGGCTGT | |
| Tenogenic | SCX | ACAGATCTGCACCTTCTGCC | GCCACCTCCTAACTGCGAAT |
| TNMD | TCCCTCAGGCTCTGGTATGA | AGGACTGAGAGACCACTGCT | |
| DCN | TGCCAAAGGATCTTCCCCCT | AGGTGTAAATGCTCCAGGACT | |
| COL1A1 | AGTGGTTTGGATGGTGCCAA | GCACCATCATTTCCACGAGC | |
| COL3A1 | TGGAGGATGGTTGCACGAAA | ACAGCCTTGCGTGTTCGATA | |
| TNC | ATGGGCAGACGCACCATTAG | TGTGCATCGACCTTCACAAGA | |
| Internal control | 18S rRNA | GTAACCCGTTGAACCCCATT | CCATCCAATCGGTAGTAGCG |
*NCBI/ Primer-BLAST: Finding primers specific to your PCR template (using Primer3 and BLAST). Available from: https://www.ncbi.nlm.nih.gov/tools/primer-blast/ (Assessed on 15 December 2017)