Fig. 4.

FLOT2 was a direct target gene of miR-143-3p and negatively regulated by miR-143-3p in melanoma cells. a The potential targeted gene of miR-143-3p was predicted by starBase v2.0. b, c The association between miR-143-3p and FLOT2 was investigated by dual-luciferase reporter assay. d, e The binding efficiency of miR-143-3p and FLOT2 to Ago2 or IgG antibody in A2058 and A375 cells was detected by RIP assay and qRT-PCR assay. f The mRNA expression of FLOT2 in melanoma tissues and normal tissues was measured by qRT-PCR. g The correlation between miR-143-3p and FLOT2 was analyzed by Spearman’s correlation coefficient analysis. h The protein level of FLOT2 in melanoma tissues and normal tissues was determined by western blot assay. i, j The mRNA and protein levels of FLOT2 in HEMn, A2059, and A375 cells were measured by qRT-PCR assay and western blot assay, respectively. k, l The levels of miR-143-3p and FLOT2 protein in A2058 and A375 cells transfected with anti-miR-NC, anti-miR-143-3p, miR-NC, or miR-143-3p were determined by qRT-PCR assay and western blot assay, respectively. *P < 0.05