Fig. 5.

MiR-143-3p suppressed melanoma cell proliferation, invasion, and glycolysis and facilitated apoptosis via binding to FLOT2. A2058 and A375 cells were divided into 5 groups: control, miR-NC, miR-143-3p, miR-143-3p + pcDNA, and miR-143-3p + FLOT2. a The protein level of FLOT2 was determined by western blot assay. b, c The proliferation of A2058 and A375 cells was tested by MTT assay. d The apoptosis of A2058 and A375 cells was assessed using flow cytometry analysis. e The invasion of A2058 and A375 cells was determined by transwell assay. f, g The consumption of glucose and the production of lactate were determined using specific kits. h, i The protein levels of HK2 and PKM2 were measured by western blot assay. *P < 0.05