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. Author manuscript; available in PMC: 2020 Mar 18.
Published in final edited form as: Food Chem Toxicol. 2012 Jul 4;50(9):3280–3290. doi: 10.1016/j.fct.2012.06.051

Table 2:

COPAS measurements on wild type C. elegans after a 2-week treatment with metal chlorides and subsequent PI staining.

Compound ppm na CRF>50b TOFc st. dev EXTC st. dev
HgCl2 0 2887 0.06 969 189 848 148
HgCl2 7.8 2912 0.16 981 186 825 147
HgCl2 31.25 2934 0.81 815 195 583 134
HgCl2 125 d
HgCl2 500 d
CdCl2 0 2960 0.06 914 205 809 153
CdCl2 7.8 2932 0.13 940 157 683 118
CdCl2 31.25 2992 0.66 681 167 449 106
CdCl2 125 e
CdCl2 500 d
CuCl2 0 2856 0.07 949 194 840 160
CuCl2 7.8 2765 0.11 952 229 820 203
CuCl2 31.25 2850 0.19 968 180 822 150
CuCl2 125 2935 0.56 683 184 496 134
CuCl2 500 d
KCl 0 2920 0.07 942 192 829 147
KCl 500 2939 0.06 899 196 816 143
a

n represents the number of readings with red values greater than zero and TOF values between 199 and 1501 for each condition analyzed by COPAS in a single experiment

b

the fraction of worms in each sample with a COPAS red fluorescence value from PI greater than 50 (instrument specific units)

c

the average and standard deviation of EXT and TOF values for each sample condition

d

all animals were dead before the experiment was completed and were not analyzed by COPAS

e

CdCl2 induced agglomeration of media components at high concentrations. While some animals were still moving after two weeks at 125 ppm CdCl2, they could not be separated from the media by centrifugation and were not analyzed.