Table 2:
COPAS measurements on wild type C. elegans after a 2-week treatment with metal chlorides and subsequent PI staining.
Compound | ppm | na | CRF>50b | TOFc | st. dev | EXTC | st. dev |
---|---|---|---|---|---|---|---|
HgCl2 | 0 | 2887 | 0.06 | 969 | 189 | 848 | 148 |
HgCl2 | 7.8 | 2912 | 0.16 | 981 | 186 | 825 | 147 |
HgCl2 | 31.25 | 2934 | 0.81 | 815 | 195 | 583 | 134 |
HgCl2 | 125 | d | |||||
HgCl2 | 500 | d | |||||
CdCl2 | 0 | 2960 | 0.06 | 914 | 205 | 809 | 153 |
CdCl2 | 7.8 | 2932 | 0.13 | 940 | 157 | 683 | 118 |
CdCl2 | 31.25 | 2992 | 0.66 | 681 | 167 | 449 | 106 |
CdCl2 | 125 | e | |||||
CdCl2 | 500 | d | |||||
CuCl2 | 0 | 2856 | 0.07 | 949 | 194 | 840 | 160 |
CuCl2 | 7.8 | 2765 | 0.11 | 952 | 229 | 820 | 203 |
CuCl2 | 31.25 | 2850 | 0.19 | 968 | 180 | 822 | 150 |
CuCl2 | 125 | 2935 | 0.56 | 683 | 184 | 496 | 134 |
CuCl2 | 500 | d | |||||
KCl | 0 | 2920 | 0.07 | 942 | 192 | 829 | 147 |
KCl | 500 | 2939 | 0.06 | 899 | 196 | 816 | 143 |
n represents the number of readings with red values greater than zero and TOF values between 199 and 1501 for each condition analyzed by COPAS in a single experiment
the fraction of worms in each sample with a COPAS red fluorescence value from PI greater than 50 (instrument specific units)
the average and standard deviation of EXT and TOF values for each sample condition
all animals were dead before the experiment was completed and were not analyzed by COPAS
CdCl2 induced agglomeration of media components at high concentrations. While some animals were still moving after two weeks at 125 ppm CdCl2, they could not be separated from the media by centrifugation and were not analyzed.