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pDNA release from PEG-b-CPCL-70:pDNA nanocomplexes at a 200:1 mass ratio (N/P = 142) by increasing polyanionic heparin. The nanocomplexes were incubated for 1 h at 37 °C with different amounts of heparin. (1)–(10) nanocomplex:heparin at mass ratios of (1) 1:0, (2) 1:0.001, (3) 1:0.002, (4) 1:0.004 (5) 1:0.006, (6) 1:0.008, and (7) 1:0.01, (8) 1:0.02, (9) 1:0.04, (10) 1:0.06, (11) pDNA, and (12) MW marker (DNA ladder).
