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. 2020 Mar 11;11:424. doi: 10.3389/fimmu.2020.00424

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Copyright © 2020 Mastroianni-Kirsztajn, Hornig and Schlumberger.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MPO-ANCA fluorescence pattern on the EUROIMMUN EUROPLUS™ Granulocyte Mosaic (IgG). (A) Examplary microscope slide with 10 reaction fields, each containing 6 biochips forming a mosaic. (B) In the EUROPLUS™ Granulocyte Mosaic, each biochip represents a different substrate: ethanol-fixed granulocytes [granulocytes (EOH)], formalin-fixed granulocytes [granulocytes (HCHO)], HEp-2 cells in combination with ethanol-fixed granulocytes [HEp-2 cells + granulocytes (EOH)] as well as PR3, MPO, and GBM microdots. The exemplary images illustrate the reactivity of a patient sample positive for anti-MPO and anti-GBM: Besides a P-ANCA pattern on ethanol-fixed granulocytes and a granular C-ANCA pattern on formalin-fixed granulocytes, MPO-ANCA is characterized by a positive fluorescence signal on MPO but not on PR3 microdots. In addition a positive fluorescence signal on GBM microdots is shown.