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Key mutations in the evolved populations.
| Locus tag | Gene | Mutation (Type) | AA change | Description |
| ELIM_c1031 | – | −356T (insertion) | Asn119LysfsX133 | Integrase family protein |
| ELIM_c1038 | – | G133A (SNV) | Glu48Lys | Putative ATPase, transposase-like protein |
| ELIM_c1073 | dam | T408G (SNV) | Tyr136X | N6 adenine-specific DNA methylase D12 class |
| ELIM_c1653 | acsA | C290A (SNV) | Ala97Glu | CODH catalytic subunit |
| ELIM_c1654 | cooC2 | −216A (insertion) | Ala72AlafsX92 | CODH nickel insertion accessory protein |
