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. 2019 Oct 8;100(11):1523–1529. doi: 10.1099/jgv.0.001334

Fig. 3.

Fig. 3.

Multistep growth-curve analysis in HUH7 cells. HUH7 cell monolayers were inoculated at an m.o.i. of 0.1, with previously described Hu/Florida/USA-2_KSA_2014 or novel deletion mutants Hu/Riyadh_KSA_2014 or Hu/Riyadh_KSA_4050_2015, and cell and supernatants were collected separately every 12 h for 84 h. Cell and supernatant fractions were titred on Vero cells.