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. 2018 Aug 1;99(10):1381–1390. doi: 10.1099/jgv.0.001130

Table 1. Primer sets used for the characterization of the 3′ 9 kb of the viral genome of rIBVs.

Primer set Primer Sequence (5′ → 3′) Amplicon (bp)a
A IBV.F12 TGTCAAGATGTCAACTGG 2327
IBV.R12 GCATTCACTGCTGTACAG
B IBV.F13 ACAGAGCACAAGTTTGATC 1721
IBV.R13 CGCTCTTAGTAACATAAAC
C IBV.F14 TAAATGGTGATCTTGTTT 2299
IBV.R14 AACACTATACCATTAGGTGC
D IBV.F15 TGCTGCTTCCTTTAATAAG 2180/2007a/1985b
IBV.R37 GAGAAAGCACCATTGGCACA
E IBV.F16 CTTAACATTGCAGTAGGTG 2361/2167c/2285d
IBV.R16 CTGAGGTCAATGCCTTATC
F IBV.F28 TGTTGTAGGTTGTGGTCCCA 2171
IBV.R31 CTAATGGGCGTCCTAGTGCT

a, amplicon length for rIBV-Δ3a.

b, amplicon length for rIBV-Δ3b.

c, amplicon length for rIBV-Δ5a.

d, amplicon length for rIBV-Δ5b.