Table 1. Primer sets used for the characterization of the 3′ 9 kb of the viral genome of rIBVs.
Primer set | Primer | Sequence (5′ → 3′) | Amplicon (bp)a |
---|---|---|---|
A | IBV.F12 | TGTCAAGATGTCAACTGG | 2327 |
IBV.R12 | GCATTCACTGCTGTACAG | ||
B | IBV.F13 | ACAGAGCACAAGTTTGATC | 1721 |
IBV.R13 | CGCTCTTAGTAACATAAAC | ||
C | IBV.F14 | TAAATGGTGATCTTGTTT | 2299 |
IBV.R14 | AACACTATACCATTAGGTGC | ||
D | IBV.F15 | TGCTGCTTCCTTTAATAAG | 2180/2007a/1985b |
IBV.R37 | GAGAAAGCACCATTGGCACA | ||
E | IBV.F16 | CTTAACATTGCAGTAGGTG | 2361/2167c/2285d |
IBV.R16 | CTGAGGTCAATGCCTTATC | ||
F | IBV.F28 | TGTTGTAGGTTGTGGTCCCA | 2171 |
IBV.R31 | CTAATGGGCGTCCTAGTGCT |
a, amplicon length for rIBV-Δ3a.
b, amplicon length for rIBV-Δ3b.
c, amplicon length for rIBV-Δ5a.
d, amplicon length for rIBV-Δ5b.