Table 2. Plasmids used for generation of the donor plasmids p-IBV-Δ3a, p-IBV-Δ3b, p-IBV-Δ5a and p-IBV-Δ 5b.

Plasmid	Genes	Coordinates	Length (nt)	3′-end RES	Surrounding RES	Inserted in plasmid
p-IBV-5	5′-UTR	1…497	497	BstBI	n.a.	n.a.
p-IBV-1b	1b, S	19 610…20 379	770	XhoI	BstBI	p-IBV-5
p-IBV-S	S	20 379…23 590	3211	StyI	XhoI	p-IBV-5-1b
p-IBV-SIR	S, 3ab, E, M	23 591…25 318	1728	EcoRI	StyI	p-IBV-5-1b-S
p-IBV-3T	5ab, N, 3′-UTR	25 319…27 730	2422	MssI, PacI	EcoRI	p-IBV-5-1b-S-SIR
p-Δ3a	n.a.	23 604…24 304	528	n.a.	NheI, PmlI	p-IBV-5-1b-S-SIR
p-Δ3b	n.a.	23 604…24 304	524	n.a.	NheI, PmlI	p-IBV-5-1b-S-SIR
p-Δ5a	n.a.	25 467…25 974	314	n.a.	AfeI, NheI	p-IBV-3T
p-Δ5b	n.a.	25 467…25 974	433	n.a.	AfeI, NheI	p-IBV-3T