Table 1. ; Pathogens tested for cross-reactivity with the RealStar® Middle East respiratory syndrome coronavirus (N Gene) reverse transcription-PCR kit 1.0.
| Pathogen | Material | Catalog number (provider) | Concentration (provider) | Concentration tested |
|---|---|---|---|---|
| Adenovirus | Genomic DNA | VR-1 (ATCC) | TCID50 ≥103.0 per 0.2 ml | Dilution 1:1000 |
| Bordetella pertussis | Genomic DNA | DSM 5571 (DSMZ) | 79.9 ng/μl | Dilution 1:200 |
| Chlamydophila pneumoniae | Genomic DNA | DSM 19748 (DSMZ) | 112.5 ng/μl | Dilution 1:1000 |
| Haemophilus influenzae | Genomic DNA | DSM 4690 (DSMZ) | 253.5 ng/μl | Dilution 1:1000 |
| hMPV | Genomic RNA | 0810164CFHI (Zeptometrix, hMPV 27 Type A2) | TCID50 ≥106.10 U/ml | Eluate 1:10 diluted |
| Human coronavirus NL63 | Genomic RNA | University Bonn | Not available | Dilution 1:10 |
| Human coronavirus 229E | Genomic RNA | University Bonn | Not available | Dilution 1:10 |
| Human coronavirus OC43 | Genomic RNA | University Bonn | Not available | Dilution 1:10 |
| Human coronavirus HKU1 | Genomic RNA | University Bonn | Not available | Dilution 1:10 |
| Influenza A virus | Genomic RNA | 0810252CFHI (Zeptometrix) | Not available | Dilution 1:10 |
| Influenza B virus | Genomic RNA | 0810255CFHI (Zeptometrix) | Not available | Dilution 1:10 |
| Klebsiella pneumoniae | Genomic DNA | 33495 (ATCC) | Not available | Eluate 1:10 diluted |
| Legionella pneumophila | Genomic DNA | DSM 7513 (DSMZ) | 163 ng/μl (diluted 1:500) | Dilution 1:500 |
| Mycobacterium tuberculosis | Genomic DNA | 25618D-2 | ≥2 μg/46 μl | Dilution 1:100 |
| Mycoplasma pneumoniae | Genomic DNA | DSM 22911 (DSMZ) | Not available (diluted 1:10) | Dilution 1:100 |
| Parainfluenza virus | Genomic RNA | VR-94 (ATCC) | TCID50 ≥103.0 per 0.2 ml | Dilution 1:100 |
| Respiratory syncytial virus | Genomic RNA | VR-26D (ATCC) | 600 ng per 100 μl | Dilution 1:10 |
| Rhinovirus | Genomic RNA | NCPV (0112169) | Not available | Dilution 1:1000 |
| SARS-CoV | Genomic RNA | University Bonn | Not available | Dilution 1:10 |
| Streptococcus pneumoniae | Genomic DNA | DSM 20566 (DSMZ) | 260 ng/μl (diluted 1:1000) | Dilution 1:1000 |
| Streptococcus pyogenes | Genomic DNA | DSM20565 (DSMZ) | Not available | Dilution 1:10 |
ATCC: American-type culture collection; CoV: Coronavirus; DSMZ: Deutsche Sammlung von Mikroorganismen und Zellkulturen.