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ELISA assay to screen inhibitors for activity against WEE1 and WEE2 in vitro. Antibodies bind phosphorylated CDC2 and undergo a chromogenic reaction to provide a semi-quantitative measurement of WEE phosphorylating activity in the presence of 1 μM of inhibitor. Error bars represent the standard error mean. Significant changes in WEE2 activity by an inhibitor compared to the no inhibitor control determined by Student’s t-test.
