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ELISA with 1 μM analogs to compound 2. Assay detects phosphorylated CDC2 as a measurement of functional inhibitory activity in vitro against WEE1 and WEE2. Error bars represent the standard error mean. Significant changes in WEE2 activity by an inhibitor compared to no inhibitor control determined by Student’s t-test with * p<0.01 and **p<0.005.
