Fig. 2.

SSc inhibits HBV replication and transcription. a HepG2.2.15 cells were treated with 20 μg/mL of SSc or 1% DMSO (v/v) for 48 h. HBV DNA were purified and quantified by real-time PCR assay. b Expression of HBsAg and HBeAg in response to SSc treatment was measured by a commercially available Elisa kit as described in Materials and methods. c Evaluation of pgRNA synthesis in response to various concentrations of SSc by real-time PCR assay with DMSO as a negative control. d HBV cccDNA purified in SSc incubated cells was quantified by real-time PCR as described in “Materials and methods”. e HepG2.2.15 cells incubated with 0, 10 and 20 μg/mL of SSc for 48 h were lysed and HBcAg production was analyzed by western blot. f Total HBV RNA extracted from SSc treated HepG2.2.15 cells was quantified by real-time PCR assay