Fig. 3.

SSc downregulates HBV pgRNA synthesis through HNF1α and HNF4α. a Expression of HNF1α and HNF4α in HepG2 and HepG2.2.15 cells was examined by western blot. b HepG2 cells transfected with pEGFP or pHBV1.3 plasmid was lysed and expression of HNF1α and HNF4α was detected by western blot. c and d HNF1α and HNF4α expression in response to SSc treatment was examined by real-time PCR and western blot assay. e–g Knockdown of HNF1α or HNF4α by specific siRNAs significantly suppressed anti-HBV activity of SSc