Fig. 4.

IL-6 downregulates expression of HNF1α and HNF4α in HepG2.2.15 cells. a Expression levels of IL-6, TGF-β, IL-1β, TNF-α and IFN-γ in response to SSc was examined by real-time PCR assay. b IL-6 expression was confirmed by dot botting with HBsAg as an internal control. c JNK phosphorylation in SSc incubated HepG2.2.15 cells were analyzed by western blot. d and e Transcription of HBV pgRNA and total RNA in response to recombinant IL-6 (rIL-6) was analyzed by real-time PCR. f Expression of HNF1α and HNF4α in SSc incubated HepG2.2.15 cells was measured by western blot. DMSO was used as a negative control