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SDS-PAGE analysis of the supernatant of BL21(DE3) containing pET28a/SUMO–scFv induced at different temperatures. Lane 1: LMW marker of protein; lane 2: supernatant of BL21(DE3) containing pET28a/SUMO–scFv induced at 37°C for 4 h; lane 3: supernatant of BL21(DE3) containing pET28a/SUMO–scFv induced at 30°C for 4 h; lane 4: supernatant of BL21(DE3) containing pET28a/SUMO–scFv induced at 18°C for 4 h; lane 5: supernatant of BL21(DE3) containing pET28a/SUMO–scFv induced at 18°C for 24 h
