Table 2.
N-terminal sequence comparison of large lima bean Limenin with related leguminous trypsin-chymotrypsin inhibitors
| Source | Name | Residue number | Residue number | % Identity | |
|---|---|---|---|---|---|
| Large lima bean (P. limensis) | Kunitz-type trypsin inhibitor | 1 | DFVIDNEGNPLENGG | 15 | 100 |
| Wild soybean (G. soja) | Kunitz-type trypsin inhibitor | 26 | DFVLDNEGNPLENGG | 40 | 93 |
| Dull black soybean (G. max) | Kunitz-type trypsin inhibitor | 1 | DFVI DNEGNPIEDGG | 15 | 87 |
| Yellow soybean (G. max) | Trypsin inhibitor subtype B | 26 | DFVLDNEGNPLDSGG | 40 | 80 |
| Soybean (G. max) | Kunitz-type trypsin inhibitor | 26 | DI VFDTEGNPI RNGG | 40 | 67 |
| Winged bean (P. tetragonolobus) | Chymotrypsin inhibitor from | 2 | DDLVDAEGNLVENGG | 16 | 60 |
| Winged bean (P. tetragonolobus) | Acidic trypsin inhibitor | 1 | ZELVDVEGKTVLNGG | 15 | 40 |
| Winged bean (P. tetragonolobus) | Basic trypsin inhibitor | 1 | EPLLDSEGELVRNGG | 15 | 40 |
Residue number 1 and residue number 15 for lima bean Limenin refer to D and G being the 1st and 15th amino acid residue in Limenin, respectively. Underlined characters, identical amino acids with the purified Limenin from the large lima