Fig. 2.

Phylogenetic tree for Chiropteran TLR8. Neighbor-joining tree was estimated based on a distance matrix of Kimuras two-parameter evolutional model using the human TLR8 sequence (XM_005274543) as outgroup to root the tree. Bootstrap percent probabilities were based on 500 replicates. The scale bar represents a genetic distance of 0.02. Estimated lineages evolving under episodic positive selection by the branch-site REL analyses are shown in red and mean ω values are represented. Amino acid sites under episodic positive selection detected singularly by MEME analyses are mapped above branches (arrows); see also Table 4