Fig. 1.

Rice expression vector pMYV733 and genomic DNA PCR analysis. The synthetic COE gene (sCOE) was fused with the M cell-target peptide ligand, Co1, introduced into a plant expression vector under the control of a signal peptide (3Dsp), promoter (RAmy3D-p), and untranslated region (3′-UTR) of a rice amylase 3D gene, and transformed into rice calli. The hygromycin phosphotransferase (HPT) gene was used as a selection marker under the control of Cauliflower mosaic virus 35S promoter (35S-p) and terminator (35S-t). LB and RB are left and right border of T-DNA, respectively (a). Genomic DNA PCR amplification was conducted to amplify the sCOE–Co1 fusion gene in genomic DNA of wild-type and putative transgenic rice calli (b). Lane M is a 100 base-pair DNA ladder (ELPIS Biotech, Seoul, Korea). Lane PC is PCR products amplified from plasmid pMYV733 used as a positive control. Lane NC is a wild type used as a negative control. Lanes 1–14 are independent putative transgenic lines