Table 2.

Overview on virus titers and virus stability of different viruses produced in MDCK or Vero cells in ΔSC and SF medium in small-scale cultivations

Virus	Cells	Medium	moi	max HA value/ time pi a	max TCID50 value/ time pi b	t c1/2	k dvi
A/PR/8/34 H1N1ma (RKI)	MDCK	ΔSC	1	3.2/89	7.6 × 107/18	19.9	−0.035
A/PR/8/34 H1N1va (RKI)	Vero	ΔSC	1	2.6/121	7.6 × 107/61	21.3	−0.033
A/PR/8/34 H1N1ma (RKI)	MDCK	SF	0.001	2.9/91	4.3 × 107/67	18.8	−0.037
A/PR/8/34 H1N1va (RKI)	Vero	SF	0.001	2.7/153	1.8 × 108/118	52.5	−0.013
A/PR/8/34 H1N1ma (NIBSC)	MDCK	ΔSC	1	2.6/64	3.2 × 106/18	12.1	−0.058
A/PR/8/34 H1N1va (NIBSC)	Vero	ΔSC	1	2.9/61	5.6 × 106/26	29.1	−0.024
A/Wis/67/2005 HGR H3N2ma	MDCK	SF	0.001	2.7/67	5.6 × 107/67	62.4	−0.011
A/Wis/67/2005 HGR H3N2va	Vero	SF	0.001	3.1/153	1.3 × 108/118	111.8	−0.006
B/Mal/2506/2004ma	MDCK	SF	0.001	2.8/91	1.8 × 108/43	9.6	−0.072

^a Maximal HA value reached (log HA units/100 μL) and time point pi for reaching maximal HA (h)

^b Maximal TCID₅₀ value reached (virions/ml) and time point pi for reaching maximal TCID₅₀ value (h)

^c Half-life time for inactivation of virus at 37 °C in roller bottles in the respective medium (h) (t_1/2 = ln2/k_vi)

^d Specific inactivation rate k_vi of virus at 37 °C in roller bottles in the respective medium (h⁻¹) determined from the slope of ln(TCID₅₀) over time during exponential decrease