Table 2.
Virus | Cells | Medium | moi | max HA value/ time pi a | max TCID50 value/ time pi b | t c1/2 | k dvi |
---|---|---|---|---|---|---|---|
A/PR/8/34 H1N1ma (RKI) | MDCK | ΔSC | 1 | 3.2/89 | 7.6 × 107/18 | 19.9 | −0.035 |
A/PR/8/34 H1N1va (RKI) | Vero | ΔSC | 1 | 2.6/121 | 7.6 × 107/61 | 21.3 | −0.033 |
A/PR/8/34 H1N1ma (RKI) | MDCK | SF | 0.001 | 2.9/91 | 4.3 × 107/67 | 18.8 | −0.037 |
A/PR/8/34 H1N1va (RKI) | Vero | SF | 0.001 | 2.7/153 | 1.8 × 108/118 | 52.5 | −0.013 |
A/PR/8/34 H1N1ma (NIBSC) | MDCK | ΔSC | 1 | 2.6/64 | 3.2 × 106/18 | 12.1 | −0.058 |
A/PR/8/34 H1N1va (NIBSC) | Vero | ΔSC | 1 | 2.9/61 | 5.6 × 106/26 | 29.1 | −0.024 |
A/Wis/67/2005 HGR H3N2ma | MDCK | SF | 0.001 | 2.7/67 | 5.6 × 107/67 | 62.4 | −0.011 |
A/Wis/67/2005 HGR H3N2va | Vero | SF | 0.001 | 3.1/153 | 1.3 × 108/118 | 111.8 | −0.006 |
B/Mal/2506/2004ma | MDCK | SF | 0.001 | 2.8/91 | 1.8 × 108/43 | 9.6 | −0.072 |
a Maximal HA value reached (log HA units/100 μL) and time point pi for reaching maximal HA (h)
b Maximal TCID50 value reached (virions/ml) and time point pi for reaching maximal TCID50 value (h)
c Half-life time for inactivation of virus at 37 °C in roller bottles in the respective medium (h) (t1/2 = ln2/kvi)
d Specific inactivation rate kvi of virus at 37 °C in roller bottles in the respective medium (h−1) determined from the slope of ln(TCID50) over time during exponential decrease