Fig. 8.

Schematic showing the detection of microRNAs using a combination of surface polyadenylation chemistry and nanoparticle amplified SPRI detection: (i) hybridization adsorption of miRNA onto a complementary LNA array element; (ii) addition of poly(A) tails to the surface bound miRNAs using poly(A) polymerase; and (iii) hybridization adsorption of T30-coated Au nanoparticles to poly(A) tails detected by SPRI measurements. Reprinted (adapted) with permission from (Fang S, Lee HJ, Wark AW, Corn RM (2006) Attomole microarray detection of micrornas by nanoparticle-amplified SPR imaging measurements of surface polyadenylation reactions. J Am Chem Soc 128(43):14044–14046). Copyright (2006) American Chemical Society [119]