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. 2010 Dec 4;63(3):155–166. doi: 10.1007/s00251-010-0498-0

Table 1.

Primers used to amplify MHC class II alleles

Locus Primer name Sequence (5′ to 3′) Tm (°C) Product (bp)
DOB DOB-F GTAGCATTATTTCCCTTT 50 600
DOB-R ACAGACAACCGTTTATCC
DPB1 DPB1-F1 CACAGAACTCGGTACTAGGAAA 52 700
DPB1-R1 CTCAGGAACCTCAAACCC
DPB1-F2 CCTGAGTGGGAAGATTTG 55.5 588
DPB1-R2 TCTCTCTGCTCCCATCCT
DQB1 DQB1-F1 CACTGGTGAGCGGGAACT 52 700
DQB1-R1 GGAGGCAAACGCATAAGG
DQB1-F2 CCCGCAGAGGATTTCGTG 62 250
DQB1-R2 GGCGACGATGCTCACCTC