Table 1.
Locus | Primer name | Sequence (5′ to 3′) | Tm (°C) | Product (bp) |
---|---|---|---|---|
DOB | DOB-F | GTAGCATTATTTCCCTTT | 50 | 600 |
DOB-R | ACAGACAACCGTTTATCC | |||
DPB1 | DPB1-F1 | CACAGAACTCGGTACTAGGAAA | 52 | 700 |
DPB1-R1 | CTCAGGAACCTCAAACCC | |||
DPB1-F2 | CCTGAGTGGGAAGATTTG | 55.5 | 588 | |
DPB1-R2 | TCTCTCTGCTCCCATCCT | |||
DQB1 | DQB1-F1 | CACTGGTGAGCGGGAACT | 52 | 700 |
DQB1-R1 | GGAGGCAAACGCATAAGG | |||
DQB1-F2 | CCCGCAGAGGATTTCGTG | 62 | 250 | |
DQB1-R2 | GGCGACGATGCTCACCTC |