Fig. 5.
Amino acid sequences of the recombinant AvBDs and SDS-PAGE analysis of His-tagged recombinant AvBD (His-rAvBD) proteins expressed in E. coli BL21 (DE3) cells. a Amino acid sequences of the recombinant AvBDs. The conserved six cysteines (C) are in bold. b SDS-PAGE analysis of His-tagged recombinant AvBD (His-rAvBD) proteins expressed in E. coli BL21 (DE3) cells. Lanes: 1–3, total protein from BL21 containing AvBD2, 6, and 12 without IPTG induction; lanes 4–6, total protein from BL21 containing AvBD2, 6, and 12 with IPTG induction; lanes 7–9, purified protein of AvBD2, 6, 12, with IPTG induction; M, protein molecular weight marker. IPTG isopropyl-β-d-thiogalactoside