Fig. 1.

Possible identification of microorganisms. Microorganisms (dark blue symbols) will be fixed directly to coated microarrays (possible species-specific monosaccharides; light green in a and b). Alternatively, microorganisms can be bound via species-specific antibodies (black Ys in c–e). Direct detection with species-specific labeled antibodies (black Ys in a and c), sandwich assays with species-specific antibodies (black Ys), and secondary labeled antibodies (brown Ys with red or green stars in b and d) could be performed. With the MIST technology (Angenendt et al. 2003a,b), detection could be performed by using an antibody bound enzyme (blue circle bound to Ys) which activity converts a substrate (yellow) to a detectable product (green or red circle in e)