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. Author manuscript; available in PMC: 2021 May 1.
Published in final edited form as: Biomaterials. 2020 Feb 21;241:119906. doi: 10.1016/j.biomaterials.2020.119906

Fig. 8. FerA increased the AAV9 binding and trafficking ability to target cells in vitro.

Fig. 8.

The effect of FerA on the ability of AAV9 binding and trafficking to cells was assessed in Huh7 (A and C) and HeLa (B and D) cells. For the binding assay, the complex of AAV9 with DPBS or hDys-FerA domain was incubated with suspended cells for 1 h at 4 °C. After that, cells were washed by centrifugation to get rid of unbound viruses. Finally, gDNA from virus bound to cells was extracted. The AAV9 genome copy number was measured and normalized to GAPDH. For the trafficking assay, the cells were stringently washed 3 times and incubated at 37 °C for another 2 h after the binding process. The percentage of viral gene copy number in the nucleus of total bound viruses in Huh7 (C) or Hela (D) cells were calculated. All treatments were performed in triplicate. Asterisks indicate statistical significance compared to the AAV9/luc alone treatment group (*p < 0.05).